Application

Research CategoryNeuroscience

Immunohistochemistry

Optimal working dilutions must be determined by end user.

Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15, Alexa Fluor 488 is an antibody against Tyrosine Hydroxylase for use in IH.

Research Sub CategoryNeurotransmitters & ReceptorsNeuronal & Glial Markers

Disclaimer

Alexa Fluor^® is a registered trademark of Molecular Probes, Inc.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma (2).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin conjugated to Alexa Fluor^® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.

Specificity

Tyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots in the dark for up to 6 months after date of receipt.